27
Participants
Start Date
September 1, 2023
Primary Completion Date
November 22, 2024
Study Completion Date
November 22, 2024
Stable isotope tracer consumption
Daily consumption of oral deuterium oxide (0.3 ml per kg of lean body mass) to equilibrate in body water and become slowly incorporated into muscle protein, enabling measurement of muscle protein synthesis rates
DXA scan
Whole body DXA scan to assess lean body mass for prescription of deuterium oxide tracer
Controlled diet
Participant will be asked to consume a provided 6-day diet package that will be prepared by the research team. This package will include 3 meals per day plus snacks
Saliva sample
Obtained from participants by asking them to spit into a tube each day during the study, for 11 days in total.
Blood sample
Resting venous blood sample from a forearm vein on 6 separate occasions
Muscle biopsy
A highly experienced researcher will sample a small section of muscle (\~200 mg) from the vastus lateralis muscle under local anaesthetic (5 ml, 1% Lidocaine)
Resistance exercise
participants will complete 8 sets of 10 repetitions of unilateral eccentric knee extensions on an isokinetic dynamometer.
Isometric strength test
Bilateral isometric strength will be measured using a well-established protocol on an isokinetic dynamometer.
Supplement
Consumption of 3 supplements a day for 3 days following resistance exercise. All supplements are commercially available.
School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham
University of Birmingham
OTHER