Investigation of miRNA Expression Profiles in Human Degenerated Intervertebral Disc Tissues

NAActive, not recruitingINTERVENTIONAL
Enrollment

50

Participants

Timeline

Start Date

April 11, 2025

Primary Completion Date

October 12, 2026

Study Completion Date

October 12, 2026

Conditions
Degeneration Disc IntervertebralmiRNA
Interventions
DIAGNOSTIC_TEST

surgery (any volume) and / or pharmaceuticals treatment initiated or planned or only dynamic observation, in accordance with current clinical guidelines

During surgery, degenerated intervertebral disc tissues will be collected under sterile conditions and stored at -80°C in cryotubes. miRNA expression profiles will be analyzed using rt-PCR. Candidate miRNAs are selected based on databases such as miRBase, miRTar, and miRDB.

DIAGNOSTIC_TEST

excised

Intervertebral disc tissues that are not degenerated will be collected during surgery and stored in the same manner as in the IVDD group. These samples will be used for comparative analysis of miRNA expression via rt-PCR to serve as the control baseline.

DIAGNOSTIC_TEST

Diagnostic work up

During surgery, degenerated intervertebral disc tissues will be collected under sterile conditions and stored at -80°C in cryotubes. miRNA expression profiles will be analyzed using rt-PCR. Candidate miRNAs are selected based on databases such as miRBase, miRTar, and miRDB.

Trial Locations (1)

17100

Çanakkale Onsekiz Mart University, Çanakkale

All Listed Sponsors
collaborator

Canakkale 18 Mart University Health Research and Application Hospital

UNKNOWN

lead

Çanakkale Onsekiz Mart University

OTHER

NCT07114497 - Investigation of miRNA Expression Profiles in Human Degenerated Intervertebral Disc Tissues | Biotech Hunter | Biotech Hunter