Synergistic Effects of Natural Oils and Herbal Extracts on Cholesterol Gallstone Dissolution: In Vitro Comparison With Rowachol®

NACompletedINTERVENTIONAL
Enrollment

55

Participants

Timeline

Start Date

December 15, 2023

Primary Completion Date

May 17, 2024

Study Completion Date

August 28, 2024

Conditions
Cholesterol Gallstone Dissolution
Interventions
DIETARY_SUPPLEMENT

Sesame Oil (SO) at 100 mg/mL in dissolving fluid.

Pure Sesamum indicum oil (cold-pressed, unrefined) dissolved in BioIVT bile juice at 100 mg/mL. Prepared by homogenization (30 min, 37°C). Used as monotherapy.

DIETARY_SUPPLEMENT

Extra Virgin Olive Oil (EVOO) at 100 mg/mL in dissolving fluid.

Pure Olea europaea oil (first cold-pressed, acidity ≤0.8%) dissolved in BioIVT bile juice at 100 mg/mL. Prepared by vortex mixing (15 min, 25°C). Used as monotherapy.

DIETARY_SUPPLEMENT

50% Sesame Oil (SO) + 50% Extra Virgin Olive Oil (EVOO).

A 1:1 (v/v) blend of Sesamum indicum oil (AIU-BC-618-1) and Olea europaea oil (AIU-BC-618-2). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by mechanical stirring (1 hr, 40°C).

DIETARY_SUPPLEMENT

50% Psyllium Extract (PE) + 50% Oil Blend (50% SO + 50% EVOO).

50% Plantago ovata mucilage extract (aqueous extraction, 65°C) + 50% AIU-BC-618-3 (SO+EVOO blend). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by sonication (20 min).

DIETARY_SUPPLEMENT

Plantago ovata extract (AIU-BC-618-4) + AIU-BC-618-3

50% Taraxacum officinale root extract (ethanol-water extraction, 70°C) + 50% AIU-BC-618-3 (SO+EVOO blend). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by magnetic stirring (30 min, 50°C).

DIETARY_SUPPLEMENT

50% Herbal Extract (25% PE + 25% DE) + 50% Oil Blend (25% SO + 25% EVOO).

25% Plantago ovata extract (AIU-BC-618-4) + 25% Taraxacum officinale extract (AIU-BC-618-5) + 25% SO (AIU-BC-618-1) + 25% EVOO (AIU-BC-618-2). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by homogenization (45 min, 37°C).

DRUG

Drug: Rowachol® (terpene extract) at 100 mg/mL in dissolving fluid.

25% Plantago ovata extract (AIU-BC-618-4) + 25% Taraxacum officinale extract (AIU-BC-618-5) + 25% SO (AIU-BC-618-1) + 25% EVOO (AIU-BC-618-2). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by homogenization (45 min, 37°C).

Trial Locations (1)

Unknown

Arab International University, Damascus

All Listed Sponsors
lead

University of Duhok

OTHER

NCT06972134 - Synergistic Effects of Natural Oils and Herbal Extracts on Cholesterol Gallstone Dissolution: In Vitro Comparison With Rowachol® | Biotech Hunter | Biotech Hunter