Analyzes of Nasal Tissue-resident Memory Immune Cells and Peripheral Memory Cells Able to Migrate to Airway Tissues (MUCOVAC)

Nasal tissue-resident memory T and B cells are quantified using flow cytometry.

Nasal fluid is collected using strip (Hunt Development) in both nostrils

Saliva is collected using Oracol (Malvern Medical Developments)

Isolation of peripheral blood mononuclear cells (PBMC) and serum from blood

ELISA to measure antibody responses in mucosal fluids and blood

Neutralization test to measure neutralizing antibody responses in mucosal fluids and blood to measure neutralizing antibody responses in mucosal fluids and blood

ELISPot to measure cellular responses in blood

Transcriptomics to quantify the gene expression of immunological markers in blood