Comparing the Dose-response Profiles of Uterotonics After Initial Carbetocin Administration - an Ex-vivo Study in Desensitized Human Myometrium

Carbetocin first bolus 10-8 M (equivalent to 100 mcg) will be added to the muscle bath to create ex-vivo environment similar to Cesarean delivery, and after 20 minutes the baths will be washed three time with physiological salt solution (PSS).

Oxytocin 10-5M will be added to all strips for 2 hours to induce desensitization.

Increasing concentrations of carbetocin in a pattern of 1 log molar increase every 10 min, from 10-5 M to 10-5 M

Increasing concentrations of oxytocin from 10-10 M to 10-5 M

Increasing concentrations of ergometrine from 10-10 M to 10-5 M

Increasing concentrations of carboprost from 10-10 M to 10-5 M