342
Participants
Start Date
January 1, 2018
Primary Completion Date
December 31, 2022
Study Completion Date
December 31, 2022
Microbiological examination of the patient's biological material and determination of antibiotic resistance
Isolated microorganisms were identified based on growth in one or more cultures obtained from punctate synovial fluid, intraoperative tissues, and from removed implants (after their ultrasonic treatment). At least 4 samples of intraoperative material (tissue biopsies, joint aspirate, removed endoprosthesis components) were taken from patients for examination. The endoprosthesis components removed during surgery were placed in a sterile plastic container and delivered to the laboratory. In the laboratory, saline solution was added to the container and processed in an ultrasonic machine according to the author's method. Identification of isolated microorganisms and sensitivity to antibiotics was carried out using an automatic analyzer and a semi-automatic analyzer using kits, test systems. Sensitivity to antibacterial drugs was tested using the disk diffusion method and analyzer kits. Antibiotic sensitivity assessment was carried out in accordance with the criteria of EUCAST.
Federal Center for Traumatology, Orthopedics and Endoprosthetics, Cheboksary
Federal State Budgetary Organization, Federal Center for Traumatology, Orthopedics and Arthroplasty
OTHER