Genetic Architecture of Neutrophil-Mediated Inflammatory Skin Diseases

DNA extraction from blood or saliva samples for the identification of gene variants by next generation sequencing;

RNA extraction from blood and skin samples for Nanostring analyses, quantitative RT-PCR or RNA sequencing.

Biobanked skin samples will be analyzed by immunostaining and imaging techniques to identify cell types in lesions and compare them to healthy skin.

Cells isolated from biobanked blood and skin samples will be cultured in vitro and proteins will be analyzed by ELISA (secreted proteins) and western blot (cell proteins).