5,340
Participants
Start Date
October 20, 2020
Primary Completion Date
December 31, 2021
Study Completion Date
December 31, 2021
SARS-CoV-2 specific IgM and IgG detection
Detection of IgM and IgG antibodies specific to the SARS-CoV-2 antigens in the blood serum using enzyme-linked immunosorbent assay (ELISA).
ELISpot: detection of the T cells specific to different SARS-CoV-2 proteins
Detection of peripheral blood T lymphocytes which are activated and secrete interferon gamma (IFNgamma) upon stimulation with peptides, covering the immunodominant sequence domains of the nucleocapsid N, membrane M, or spike glycoprotein S proteins of SARS-CoV-2 coronavirus.
Flow cytometry: detection of the SARS-CoV-2 specific T-helpers and cytotoxic T lymphocytes
Detection of peripheral blood T-helpers (CD45+CD3+CD4+)\* and cytotoxic T cells (CD45+CD3+CD8+)\* which are activated and secrete IFNgamma and/or interleukin-2 (IL2) upon stimulation with mixture of peptides, covering the immunodominant sequence domains of the nucleocapsid N, membrane M, and spike glycoprotein S proteins of SARS-CoV-2 coronavirus. \* CD, cluster of differentiation
Clinical City Hospital named after I.V. Davydovsky of Moscow Department of Healthcare, Moscow
Clinical City Hospital named after I.V. Davydovsky of Moscow Department of Healthcare
NETWORK
Moscow State University of Medicine and Dentistry
OTHER
Moscow Institute of Physics and Technology
UNKNOWN
National Research Center for Hematology, Russia
NETWORK
State Research Center Institute of Immunology, Russia
UNKNOWN
Shemyakin-Ovchinnikov Institute of bioorganic chemistry RAS
UNKNOWN
National Medical Research Center of Phthisiopulmonology and Infectious Diseases
OTHER_GOV
Moscow Department of Health
OTHER_GOV