54
Participants
Start Date
December 1, 2019
Primary Completion Date
May 1, 2020
Study Completion Date
September 30, 2020
DNA extraction and 16S rRNA sequencing
DNA extraction using QIAamp® DNA mini kit (QIAGEN) was done under aseptic techniques in the sterile room for all samples according to the manufacturer instructions. The DNA extracts for the 54 samples collected (27 from asthmatic patients and 27 from healthy subjects) were stored at -80° C in a sterile Eppendorf tubes until they were sent to the Molecular Research (MR DNA) Lab (Molecular Research LP, Shallowater, TX, USA) for sequencing.
Applied Science private University, Amman
Applied Science Private University
OTHER