280
Participants
Start Date
May 1, 2017
Primary Completion Date
December 31, 2025
Study Completion Date
December 31, 2025
Detection of Plasma levels of 25(OH) vitamin D by ELISA technique
Five milliliters of peripheral venous blood sample will be taken (after 8-12 hours of fasting) from each participant and will be collected into HumaTube Serum Gel - C/A for ELISA. Serum will be appropriately obtained and stored at -20°C maximum of 30 days. Plasma levels of 25(OH) vitamin D will be detected by enzyme-linked immunosorbent assay (ELISA) technique.
Detection of Total serum immunoglobulin E by ELISA technique
Five milliliters of peripheral venous blood sample will be taken (after 8-12 hours of fasting) from each participant and will be collected into HumaTube Serum Gel - C/A for ELISA. Serum will be appropriately obtained and stored at -20°C maximum of 30 days. Total serum immunoglobulin E will be detected by enzyme-linked immunosorbent assay (ELISA) technique.
Research institute of epidemiology, microbiology and infectious diseases, Tashkent
Research Institute of Epidemiology, Microbiology and Infectious Diseases, Uzbekistan
OTHER_GOV