The Microbiome as a Target for Precision Medicine in Atherosclerosis

"In patients who have been successfully revascularized the artery responsible for AMI and also present an intermediate lesion (40-80%) in another coronary territory, the clinical care protocol of the Cardiology Service stipulates the need for a physiological assessment with guidance of pressure (FFR).~The thickness of the fibrous cap shall be measured using optical coherence tomography.~In addition to the FFR measurement, a complete physiological assessment with a Doppler-pressure guide. This will allow the procedure to be performed without additional risk to the patient. The physiological study will include the analysis of endothelium-dependent vascular function and endothelium-independent vascular function."

From the blood samples of the patients, the total DNA will be extracted and the main functional variants identified in the literature will be genotyped

From the samples of blood, feces, oral cavity and blood, the DNA of the microbiota will be extracted using specific extraction kits and the microbiome will be analyzed through the study of 16S ribosomal RNA amplicons.

A study of immunological cell populations and cytokines will be carried out from fresh blood samples using antibody panels and flow cytometry

Clinical evaluation including hemostasis and biochemical studies and questionaries for diet and exercice registration