120
Participants
Start Date
April 1, 2018
Primary Completion Date
September 15, 2019
Study Completion Date
October 30, 2019
Sperm DNA integrity by flow cytometry
flowcytometric test where sperm DNA breaks are evaluated indirectly through the DNA denaturability. The assay measures the susceptibility of sperm DNA to acid-induced DNA denaturation in situ, followed by staining with the fluorescence dye acridine orange. By using a flow cytometer 5.000-10.000 sperm can be analyzed within few seconds and thus provide a less subjective result compared to the World health organization analysis where only 1-300 cells are analyzed.
Ahmed Abbas, Assiut
Assiut University
OTHER