Tissue Penetration of Ceftolozane/Tazobactam in Diabetic Patients With Lower Limb Infections

3 or more doses of ceftolozane/tazobactam intravenously administered over 60 minutes every 8 hours

A 20 kila-Dalton microdialysis probe (63 MD catheter; MDialysis Inc., N. Chelmsford, MA) will be inserted into the subcutaneous tissue at the margin of the wound (patient group) or in the thigh tissue (healthy volunteers). The probe will be left in place for the final dose and all tissue sampling procedures thereafter. This probe is perfused with a physiologic solution to collect interstitial fluid samples. The probe will then be removed after completion of sample collection.