Effects of β-alanine and Sodium Bicarbonate Supplementation on Physical Capacity and Biochemical Markers Concentrations

"Assigned Interventions:~* Beta-alanine (BA)~* Sodium bicarbonate (SB)~Description:~• Participants receive beta-alanine and sodium bicarbonate~Intervention list \& supplementation details:~1. Beta-alanine (BA)~ * Form: Gelatin capsules~ * Substance: Pure beta-alanine~ * Daily dose: 6.4 g/day~ * Dosing schedule: Four equal servings taken with meals (breakfast, lunch, snack, dinner)~ * Supplementation period: days 1 to 28~2. Sodium bicarbonate (SB)~ * Form: Gelatin capsules~ * Substance: Pure sodium bicarbonate~ * Daily dose: 0.3 g/kg body mass per day~ * Dosing schedule: Three equal servings taken with meals (breakfast, lunch, dinner)~ * Supplementation period: days 21 to 28 of BA or PL1 supplementation~Rationale:~• This group tests the combined effects of BA and SB"

"Participants in this intervention group will undergo chronic supplementation with beta-alanine (BA) and an inert placebo (PL2) matched to the sodium bicarbonate (SB) protocol.~* Beta-alanine (pure, in gelatin capsules) will be administered at a total daily dose of 6.4 g, divided into four servings taken with meals, over a period of 28 consecutive days.~* PL2 (placebo matched to SB, in gelatin capsules) will be administered from Day 21 to Day 28 of beta-alanine supplementation, at a daily dose of 0.3 g per kg of body mass, divided into three equal servings taken with meals.~This group allows for evaluation of the isolated effects of BA supplementation when combined with a placebo comparator to SB"

"Assigned Interventions:~* Placebo matched to BA (PL1)~* Sodium bicarbonate (SB)~Description:~• Participants receive only sodium bicarbonate as the active compound and placebo capsules matching the BA protocol.~Intervention list \& supplementation details:~1. Placebo matched to BA (PL1)~ * Form: Gelatin capsules~ * Substance: Inert substance matched in appearance and dosing schedule to BA~ * Daily dose: 6.4 g/day~ * Dosing schedule: Four equal servings taken with meals (breakfast, lunch, snack, dinner)~ * Supplementation period: days 1 to 28~2. Sodium bicarbonate (SB)~ * Form: Gelatin capsules~ * Substance: Pure sodium bicarbonate~ * Daily dose: 0.3 g/kg body mass per day~ * Dosing schedule: Three equal servings taken with meals (breakfast, lunch, dinner)~ * Supplementation period: days 21 to 28 of BA or PL1 supplementation~Rationale:~• This group tests the isolated effects of SB"

"Assigned Interventions:~* Placebo matched to BA (PL1)~* Placebo matched to SB (PL2)~Description:~• Participants receive two matched placebos.~Intervention list \& supplementation details:~1. Placebo matched to BA (PL1)~ * Form: Gelatin capsules~ * Substance: Inert substance matched in appearance and dosing schedule to BA~ * Daily dose: 6.4 g/day~ * Dosing schedule: Four equal servings taken with meals (breakfast, lunch, snack, dinner)~ * Supplementation period: days 1 to 28~2. Placebo matched to SB (PL2)~ * Form: Gelatin capsules~ * Substance: Inert substance matched in appearance and timing to SB~ * Daily dose: 0.3 g/kg body mass per day~ * Dosing schedule: Three equal servings taken with meals (breakfast, lunch, dinner)~ * Supplementation period: days 21 to 28 of BA or PL1 supplementation~Rationale:~• This serves as the control group for comparison with all active intervention arms."