Discovery and Analytical Validation of Inflammatory Bio-signatures of the Human Pain Experience

intravenous injection of 100 milligrams anakinra

intravenous injection of 1 milliliter normal saline

The experimental, standardized, nociceptive pain challenge will induce a moderate level of sustained pain. The pain challenge involves a masseteric injection in the left or right jaw muscle of normal saline (0.15 ml bolus of 0.9% normal saline) over a 15 second period followed by continuous infusion via a closed loop infusion system for 20 minutes.