Body Fat as Determinant of Female Gonadal Dysfunction

"* Weight and height.~* Waist-to-hip ratio.~* Body composition: Bioelectrical impedance and \[Dual energy X-ray absorptiometry (DEXA)\]."

Energy availability assessment.

"* Lipid profile.~* Oral glucose tolerance test: plasma glucose and insulin, insulin sensitivity indices, gastrointestinal hormones, adipokines, oxidative stress markers.~* Sex steroid profile.~* Hypothalamic-pituitary-adrenal axis study.~* Ferrokinetic study.~* Subclinical chronic inflammatory markers."

"* Polycystic ovarian morphology.~* Carotid intima-media thickness.~* Eco-FAT: Ultrasound measurements of adipose tissue depots including sc, preperitoneal, intraperitoneal (ip), mesenteric, and perirenal fat thickness."

A\&D TM2430EX oscillometric devices (A\&D Company Limited, Tokyo, Japan).

Subcutaneous fat tissue and muscle tissue for proteomics an gene expression studies.

Parasympathetic and sympathetic responses to deep breathing, Valsalva's maneuver and orthostatism.

Microbiome studies.