The Effects of Acute Total Sleep Deprivation Versus Normal Sleep on Metabolism

Participants perform a binary decision on each presented stimuli. Of the two possible outcomes, participants are instructed to make a motor response (go) for one type, and are to withhold a response (no-go) for the other type. Reaction time and accuracy are measured for each event

Hormone levels, neuromolecular levels and gene expression profiles will be analyzed from repeated blood samples obtained before and after the nighttime intervention

Expression profiles will be analyzed from samples obtained from tissues involved in metabolism

75 g of glucose will be dissolved in 300 ml of water and given to participants, followed by blood sampling at 0, 15, 30, 60, 90, 120 and 150 minutes following the ingestion of the glucose solution.

Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume