Chemotherapy Followed by Allogeneic Stem Cell Transplantation for Hematologic Malignancies

"Donor: Prior to mobilization, leukapheresis to collect CD3+ cells. The donor will then receive G-CSF (10 mcg/kg/day) with leukapheresis collection of peripheral blood stem cells on days 5, 6 and 7 as needed. Goal of leukapheresis will be \> 5 x 106 CD34+cells/kg of recipient.~Patient: Peripheral Blood Stem Cell (PBSC) Transplant. Fludarabine 25mg/m2/d IV over 30 minutes on days -6 to -2, followed by cyclophosphamide 1g/m2/d IV on days -3 and -2. This will be followed by allogeneic stem cell infusion 48 hours later.~Donor Lymphocyte Infusion (DLI) and Adjustment of Immunosuppression: Cyclosporine (CSA) and methotrexate (MTX) will be used for GvHD prophylaxis with target CSA levels of 200-400 ng/ml."

10 mcg/kg/day on days 5, 6, and 7

25 mg/m2/d IV over 30 minutes on days -6 to -2

1 g/m2/d IV on days -3 and -2

used for GvHD prophylaxis with target CSA levels of 200-400 ng/ml

used for GvHD prophylaxis with target CSA levels of 200-400 ng/ml